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2026-11
90/100 stars
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Image Search Results
Journal: Journal of Histochemistry and Cytochemistry
Article Title: Hyperglycemia Alters the Protein Levels of Prominin-1 and VEGFA in the Retina of Albino Rats
doi: 10.1369/0022155417737484
Figure Lengend Snippet: Light and EM photographs of the retina of control and diabetic rats. Panels A and B: Control retinas show normal capillaries with intact pericytes (arrowhead) and flat endothelial cells (arrow) resting on a regular basement membrane (curved arrow). Panels C–F: Diabetic retinas show a capillary within the GCL with an irregularly thickened basement membrane (curved arrow). Notice the decreased number of cells in the GCL. Acellular blood vessels, endothelial cell damage, and pericyte loss (thick arrows) are also present. Panel G: Control retinas show weak expression of CASP-3 in the GCL, IPL, and OPR. Panel H: Diabetic retinas showed higher expression of CASP-3 in all retinal layers compared with control retinas. A, C, and E = toluidine blue; G and H = CASP-3 immunoperoxidase; B (TEM magnification = 25,000×), D (TEM magnification = 6000×), and F (TEM magnification = 8000×), uranyl acetate and lead citrate. Abbreviations: EM, electron microscopy; GCL, ganglion cell layer; IPL, inner plexiform layer; OPR, outer segment of the photoreceptor; TEM, transmission electron microscopy. Scale bar = 50 µm.
Article Snippet: After incubation for 10 min in a blocking solution (10× phosphate-buffered saline [PBS] and 2% normal horse serum) to control background staining (Histo-Plus kit; Zymed, San Francisco, CA), sections were incubated for 30 min with primary
Techniques: Expressing, Electron Microscopy, Transmission Assay